Journal: PLOS Pathogens
Article Title: Entry, replication and innate immunity evasion of BANAL-236, a SARS-CoV-2-related bat virus, in Rhinolophus and human cells
doi: 10.1371/journal.ppat.1013573
Figure Lengend Snippet: (A-P) Caco-2 and RFe-ATC cells were infected for 72 h. Caco-2 cells were infected with SARS-CoV-2 (A-D) or BANAL-236 (I-L) at an MOI 0.001 and 0.01 respectively. RFe-ATC cells were infected with SARS-CoV-2 (E-H) and BANAL-236 ( M-P ) at MOI 0.02 and 0.2 respectively. Cells were then subjected to transmission electron microscopy analysis. DMV-like structures are pointed by white arrows. (Q-R) At 48 and 72 h post-infection supernatants from Caco-2 (Q) and RFe-ATC (R) cells were collected. Titration of clarified supernatants by TCID 50 assays was performed on Vero-E6 cells infected for 5 days. Data are the means ± SEM of at least two independent experiments.
Article Snippet: Human colorectal adenocarcinoma Caco-2 cells (kind gift from Nathalie Sauvonnet, Institut Pasteur, Paris), African green monkey kidney epithelial Vero-E6 cells (ATCC CRL-1586), A549 lung cancer cells (ATCC CCL-185), and human embryonic kidney (HEK) 293T cells (ATCC CRL-3216), hereinafter referred to as 293T, were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) containing GlutaMAX I, sodium pyruvate (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Dutscher) and 1% penicillin and streptomycin (10 000 IU/ml; Thermo Fisher Scientific).
Techniques: Infection, Transmission Assay, Electron Microscopy, Titration